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Ras and PI 3-kinase activation of Pak in Rat-1 cells. Rat-1 cells were transfected with the indicated plasmids along with a myc-tagged Pak1 construct; extracts were then prepared and used in immune kinase assays. Fold indicates fold activation compared to a vector control (lane 1) as determined by PhosphorImager analysis. MBP, myelin basic protein.
