Figure 6. IL-4 and IL-13 dependence of muscle cell induced fusion of cancer cells.
(A) Fluorescence microscopy images of PC3 cells grown in the presence of either recombinant IL-4 (50 ng/ml), recombinant IL-13 (50 ng/ml) or neither for 72 h. Arrows mark examples of the co-labeled fused cells. Bar, 25 μm. (B, C) Quantification of content mixing between PC3 cells (B) and between pPCa cells (C) grown in the presence of IL-4 or IL-13 or neither. (D) Multinucleation for pPCa cells grown with and without IL-4 and IL-13. (E, F) Western blot analysis of expression of tubulin (as a loading control) and Syn1 and AnxA5 for PC3 cells and PC3 cells grown in the presence of 50 ng/ml IL-4 (E) or IL-13 (F). (G) Percentage of multinucleated cells for PC3 cells (1), PC3 cells co-cultured in a direct contact with mMYO and non-specific IgG (2), and PC3 co-cultured with mMYO in the presence of neutralizing antibody against IL-4 (3) or IL-13 (4). All results are shown as means ± SEM (n ≥ 3).