Figure 3. NEAT1 directly binds to miR-34a and negatively regulates miR-34a.
(A) Five binding sites between NEAT1 and miR-34a were analyzed from the bioinformatics database (starbase.sysu.edu.cn). (B) The Pearson’s correlation analysis revealed a negative correlation between NEAT1 and miR-34a expressions from cervical cancer tissues. (C) NEAT1 overexpression vector or (D) NEAT1 shRNA as well as control vector was transfected into CaSki and SiHa cells for 48 h, followed by measurements of miR-34a expressions by qRT-PCR. GAPDH and U6 were used as internal control for lncRNA and miRNAs, respectively. All data were shown as mean ± S.D. *, P<0.05; **, P<0.01.