Fig. 2.
The western blot, LDH enzyme activity and H2O2 production rate in HeLa and 4T1 cells. a Western blot confirmation of LDH knockout in HeLa LDH knockout cell lines. b Specific LDH enzyme activity assayed by converting pyruvate to lactate in HeLa LDH knockout cells. c Relative H2O2 production rate in HeLa LDH knockout cells. d Western blot confirmation of LDH re-expression in HeLa LDH knockout cell lines. e Specific LDH enzyme activity assayed by converting pyruvate to lactate in HeLa LDH knockout and re-expression cells. f Relative H2O2 production rate in HeLa LDH knockout and re-expression cells. g Western blot confirmation of LDH knockout in 4T1 LDH knockout cell lines. h Specific LDH enzyme activity assayed by converting pyruvate to lactate in 4T1 LDH knockout cells. i Relative H2O2 production rate in 4T1 LDH knockout cells. Data were confirmed by at least three independent experiments. The experimental details are described in Materials and Methods