Figure 1.
PFK15 treatment inhibits metabolic reprogramming to glycolysis during T cell activation. NOD.BDC2.5 splenocytes were stimulated with their cognate peptide MM ± PFK15 for 24-72 hrs. (A) Schematic diagram displaying the mechanism of action of PFK15. (B) Representative western blot analysis of glycolysis proteins in untreated, MM stimulated, and MM+ PFK15 treated splenocytes after 48 hrs in culture. (C, D) Representative histogram and statistical analysis showing 2-NBDG fluorescence and MFI of CD4+ T cells, indicative of glucose uptake 48 hrs post stimulation (n = 3). (E) Lactate measurements in cell culture supernatants (n = 6). All data are presented as the mean ± SEM. (not significant (ns), *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001).