Figure 2.
Murine LST1 is expressed in the cells of myeloid lineage and its expression increases after pro-inflammatory stimuli. (A) Murine LST1 was detected in total cell lysates by immunoblotting (n=3). MF – bone marrow derived macrophages, DC – bone marrow derived dendritic cells, Neu – Neutrophils, B – B cells, T – T cells, Mono – monocytes, NK – NK cells. Its molecular weight varies between 42 to 71 kDa, likely due to the glycosylation. (B, C) Expression of LST1 in bone marrow derived macrophages (B) and dendritic cells (C) after treatment with various pro-inflammatory stimuli (n=3, see Materials and Methods for concentrations).