. Author manuscript; available in PMC: 2021 Jul 1.

Published in final edited form as: Nat Cancer. 2020 Dec 14;2(1):66–82. doi: 10.1038/s43018-020-00148-7

Table 1.

Antibodies used for CyCIF

Cycle	Channel	Antibody	Fluorophore	Manufacturer	Clone	Catalog Number	Dilution
Cycle1	FITC	anti-Rabbit-488	Alexa Fluor 488	ThermoFisher	Polyclonal	A-21246	1:2000
	Cy3	anti-Rat-555	Alexa Fluor 555	ThermoFisher	Polyclonal	A-21434	1:2000
	Cy5	anti-Mouse-647	Alexa Fluor 647	ThermoFisher	Polyclonal	A-21237	1:2000
Cycle 2	FITC	anti-GZMB	anti-Rabbit-488	Abcam	Polyclonal	AB4059	1:500
	Cy3	anti-GZMB	anti-Rat-555	eBioscience	496B	14–8889-80	1:250
	Cy5	anti-GZMB	anti-Mouse-647	Dako	GrB-7	M7235	1:200
Cycle 3	FITC	CD8a-488	Alexa Fluor 488	eBioscience	AMC908	53–0008-80	1:400
	Cy3	CD3D-555	Alexa Fluor 555	Abcam	EP4426	AB208514	1:200
	Cy5	CD45–647	Alexa Fluor 647	BioLegend	HI30	304020	1:200
Cycle 4	FITC	CD68–488	Alexa Fluor 488	CST	D4B9C	24850S	1:200
	Cy3	Foxp3–570	eFluor 570	eBioscience	236A/E7	41–4777-82	1:150
	Cy5	CD14–647	Alexa Fluor 647	Abcam	Polyclonal	ab196169	1:400
Cycle 5	FITC	CD163–488	Alexa Fluor 488	Abcam	EPR14643–36	ab218293	1:200
	Cy3	Keratin-570	eFluor 570	eBioscience	AE1/AE3	41–9003-82	1:400
	Cy5	PD1–647	Alexa Fluor 647	Abcam	EPR4877(2)	ab201825	1:200
Cycle 6	FITC	CD4–488	Alexa Fluor 488	R&D Systems	Polyclonal	FAB8165G	1:200
	Cy3	Ki67–570	eFluor 570	eBioscience	20Raj1	41–5699-80	1:200
	Cy5	PDL1–647	Alexa Fluor 647	CST	E1L3N	15005	1:200