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. 2021 Mar 16;184(9):2316–2331.e15. doi: 10.1016/j.cell.2021.03.029
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
COV2-2196 Zost et al., 2020a N/A
COV2-2130 Zost et al., 2020a N/A
COV2-2489 Zost et al., 2020a N/A
COV2-2676 Zost et al., 2020a N/A
COV2-2490 Zost et al., 2020a N/A
COV2-2263 Zost et al., 2020a N/A
CR3022 ter Meulen et al., 2006 N/A
r2D22 Smith et al., 2012 N/A
SARS2-38 (mouse monoclonal) This paper N/A
Goat anti-human lambda, mouse ads-UNLB Southern Biotech RRID: AB_2795760
Goat anti-human kappa, mouse ads-UNLB Southern Biotech RRID: AB_2795728
Goat anti-human IgG Fc, Multispecies ads-HRP Southern Biotech RRID: AB_2795580
Goat anti-human IgG-HRP Southern Biotech Cat# 2040-05, RRID: AB_2795644
Goat anti-human IgG Alexa Fluor 647 Invitrogen Cat# A-21445
SARS2-16 (mouse monoclonal) This paper Michael S. Diamond laboratory, Washington University
Bacterial and Virus Strains
VSV-SARS-CoV-2 Case et al., 2020b N/A
G142D VSV-SARS-CoV-2 (COV2-2489 escape mutant) This paper N/A
R158S VSV-SARS-CoV-2 (COV2-2489 escape mutant) This paper N/A
F140S VSV-SARS-CoV-2 (COV2-2676 escape mutant)
SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020) CDC/BEI Resources NR52281
SARS-CoV-2 (isolate B.1.1.7) Chen et al., 2021b N/A
SARS-CoV-2 (B.1.351 spike protein chimeric virus) Chen et al., 2021b N/A
Chemicals, Peptides, and Recombinant Proteins
SARS-CoV-2 S6Pecto Greaney et al., 2021 N/A
Avidin–Peroxidase Sigma Cat# A3151
1-step Ultra TMB-ELISA substrate solution Thermo Fisher Scientific Cat# 34029
ExpiCHO Expression Medium Thermo Fisher Scientific Cat# A2910001
FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 1238002
Fetal Bovine Serum, ultra-low IgG Thermo Fisher Scientific Cat# 16250078
EZ-Link™ NHS-PEG4-Biotin, No-Weigh™ Format Thermo Fisher Scientific Cat# A39259
FabALACTICA® Fab kit Genovis Cat# A2-AFK-025
BioLock biotin blocking IBA Lifescience Cat# 2-0205-050
Uranyl Formate EMS Cat# CF400-CU-50
Critical Commercial Assays
MagMAX™-96 Viral RNA Isolation Kit Thermo Fisher Scientific AM1836
MagMAX™ mirVana™ Total RNA Isolation Kit Thermo Fisher Scientific A27828
TaqMan RNA-to-Ct 1-Step Kit Thermo Fisher Scientific 4392939
Mouse Cytokine Array / Chemokine Array 31-Plex (MD31) Eve Technologies MD31
Deposited Data
COV2-2489 Fab complex with SARS-CoV-2 S6Pecto This paper EMDB: EMD-23154
COV2-2676 Fab complex with SARS-CoV-2 S6Pecto This paper EMDB: EMD-23155
Experimental Models: Cell Lines
Monkey: Vero E6 ATCC ATCC: CRL-1586, RRID: CVCL_0574
Monkey: Vero E6 +TMPRSS2 Diamond laboratory, Washington University N/A
Monkey: Vero E6 + ACE2 + TMPRS22 A. Creanga and B. Graham (Vaccine Research Center, NIH) N/A
Monkey: Vero CCL-81 ATCC CCL-81; RRID: CVCL_0059
Monkey: Vero Furin Mukherjee et al., 2016 N/A
Monkey: MA104 ATCC ATCC CRL-2378.1
Hamster: ExpiCHO-S Thermo Fisher Scientific Cat# A29127, RRID: CVCL_5J31
Human: FreeStyle 293-F Thermo Fisher Scientific Cat# R79007, RRID: CVCL_D603
Human: HEK293T ATCC ATCC CRL-3216
Human: HEK293T+ACE2 Diamond laboratory, Washington University N/A
Human: HEK293 ATCC ATCC CRL-1473
Experimental Models: Organisms/Strains
Mouse: B6.Cg-Tg(K18-ACE2)2Prlmn/J Jackson Laboratory Cat# 034860; RRID:IMSR_JAX:03486
Oligonucleotides
SARS-CoV-2 N F: 5′-ATGCTGCAATCGTGCTACAA-3′ Hassan et al., 2020 N/A
SARS-CoV-2 N R: 5′-GACTGCCGCCTCTGCTC-3′ Hassan et al., 2020 N/A
SARS-CoV-2 N Probe: 5′-/56-FAM/TCAAGGAAC/ZEN/AACATTGCCAA/3IABkFQ/-3′ Hassan et al., 2020 N/A
Gapdh TaqMan Primer/Probe set IDT Mm.PT.39a.1
CACACTTTCCTCGTGAAGG This paper VSV RNA amplification
CACTATTAAATTGGTTGGCAATC This paper VSV RNA amplification
GTACAATCACTTCTGGTTGG This paper VSV RNA amplification
GAACAGCAACCTGGTTAGAAG This paper VSV RNA amplification
CAGAGACATTGCTGACACTAC This paper VSV RNA amplification
GTCTACAGCATCTGTAATGG This paper VSV RNA amplification
GATTCTTCTTCAGGTTGGACAG This paper VSV RNA amplification
Forward-AGCTTCTGAACAATCCCCGG This paper VSV DNA Sequencing
Reverse-GAGGCCTCTTTGAGCATGGT This paper VSV DNA Sequencing
Recombinant DNA
Plasmid: pTwist-mCis_G1 COV2-2489 Zost et al., 2020a N/A
Plasmid: pTwist-mCis_G1 COV2-2676 Zost et al., 2020a N/A
Plasmid: pTwist-CMV S6Pecto Greaney et al., 2021 N/A
Plasmid: pTwist-mCis_G1 rCR3022 Zost et al., 2020a N/A
Plasmid: pTwist-mCis_G1 COV2-2196 Zost et al., 2020a N/A
Plasmid: pTwist-mCis_G1 COV2-2130 Zost et al., 2020a N/A
Plasmid: pTwist-mCis_G1 COV2-2263 Zost et al., 2020a N/A
Plasmid: pTwist-mCis_G1 COV2-2490 Zost et al., 2020a N/A
Software and Algorithms
RTCA version 2.1.0 Acea Biosciences, Inc RTCA Software, RRID: SCR_014821
Serial EM 3.7 N/A SerialEMRRID: SCR_017293
Topaz 0.2.3 Bepler et al., 2019, Bepler et al., 2020 Topaz
UCSF Chimera 1.14 Pettersen et al., 2004 UCSF Chimera, RRID: SCR_004097
Cryosparc 3.0.0 Punjani et al., 2017 RRID: SCR_016501
FlowJo FlowJo, LLC v10
GraphPad Prism GraphPad v 9.0.0
Nanozoomer Digital Pathology Hamamatsu v2
Other
xCELLigence RTCA MP analyzer Acea Biosciences, Inc N/A
xCELLigence E-Plate 96 PET cell culture plates Acea Biosciences, Inc Cat# 300601010
ÄKTA pure chromatography system GE Healthcare Life Sciences N/A
Biostack microplate stacker BioTek N/A
FEI TF20 electron microscope with Gatan US4000 4k × 4k CCD camera TFS N/A
StrepTrap HP column GE Healthcare Life Sciences Cat# 28-9075-48
400 mesh copper EM grids EMS Cat# 22451
EL406 washer dispenser BioTek N/A
HisTrap Excel column GE Healthcare Life Sciences Cat# 17-3712-06
HiTrap MabSelect™ SuRe 5 mL column GE Healthcare Life Sciences Cat# 29-0491-04