FIGURE 5.
The effects of AD, AD-soy-pea, and rodent diet after 24 wk on chronic CD-ileitis in young GF SAMP mice colonized with human gut microbiota (hGF-SAMP) (Experiments 1, 5, and 6). Values are mean ± SD, n = 5–7. (A) Fecal MPO measured 3 d after starting diets. The differences in MPO between hGF-SAMP treatment groups continued through to week 24. (B, C) Ileitis severity: (B) 3D-SM percentage abnormal mucosa (left panel) and histology scores (right panel) for terminal ilea, and (C) representative 3D-SM photographs (ileum) for hGF-SAMP and age-matched GF-SAMP. (D) FITC-dextran recovered in plasma of GF-SAMP colonized with CD or HC microbiota. (E) Metagenomic sequencing PC analysis plots for mice groups and respective human donors. Arrows show differences in the magnitude of effect between diet and donor microbiome. (F) Firmicutes:Bacteroidetes ratio across 3 different donor-mouse groups (mean ± SD). a,bLabeled means without a common letter differ (1-factor Kruskal–Wallis ANOVA with Dunn's multiple comparison test). *Different from control, P < 0.05. AD, American diet; AD-soy-p, American diet modified by protein source (soy and pea isolates); CD, Crohn disease; con, control; FITC, fluorescein isothiocyanate; GF, germ-free; HC, healthy control; hGF, “humanized” germ-free; MPO, myeloperoxidase; PC, principal component; SAMP, SAMP1/YitFC mouse line; 3D-SM, stereomicroscopy 3-D pattern profiling.