FIGURE 4.
Accumulation of Bcl-2 clone 4 T cells in InsHA mice is due to their resistance to apoptosis rather than increased islet damage. InsHA mice were treated with FTY720 on day −1, 1, 3, and 5 i.p. On day 0, clone 4 or Bcl-2 clone 4 T cells were adoptively transferred into InsHA mice. A, Seven days later, peripheral and pancreatic LNs were harvested and donor CFSE-labeled clone 4 T cells were analyzed by flow cytometry. The percentage of activated clone 4 T cells is indicated. B, Total numbers of clone 4 or Bcl-2 clone 4 T cells (from A) were quantitated by flow cytometry. Graphs represent the mean of activated (based on CFSE dilution) clone 4 or Bcl-2 clone 4 T cells in the pancreatic LNs of InsHA mice. Data are representative of three to four mice per group from one of two independent experiments with similar results.