Extended Data Fig. 1. Pull-downs of FERARI subunits show multiple interactions.
(a) Schematic drawing of the putative FERARI tether. (b) Pull-downs were performed with extracts from yeasts expressing SPE-39-FLAG and the HA-tagged proteins indicated at the top. Control pull-downs with beads coupled to IgG were also carried out. Arrows on the left indicate the sizes of expressed proteins. n=3 independent experiments. Quantification is shown in (b’); mean ± s. d. is given. (c) Pull-downs showing interactions between SPE-39-FLAG and HA-RME-1 fragments. n=3 independent experiments. Quantification is shown in (c’); mean ± s. d. is given. (d) Pull-downs experiments using SPE-39-FLAG and fragments of UNC-44 (with HA tag). UNC-44D fragment expression is low and can be seen on longer exposure as indicated. n=3 independent experiments. Quantification is shown in (d’); mean ± s. d. is given. See Unprocessed Blots Extended Data 1 and Statistical Source Data Extended Data 1.