Figure 2. The TME activates IRE1/sXBP1 in pro-tumorigenic TAMs.
a, qPCR analysis of mRNA expression of indicated genes in splenic macrophages (n=9) and TAMs (n=8) isolated from YUMM1.7 melanoma-bearing mice. b, Representative histogram (left) and quantitative plot of the percentage (right) of sXBP1+ cells among splenic macrophages and TAM of YUMM1.7 melanoma-bearing mice (n=9 per group). c, Representative histogram (left) and quantitative plot of the MFI (right) of BODIPY staining in TAMs gated based on sXBP1 expression (n=9 per group). d, Representative histogram (left) and quantitative plot of the percentage (right) of sXBP1+ cells among ARG1+ and ARG1- TAMs (n=9 per group). e, Violin plot and box plot show distributions of ER stress scores in anti-tumorigenic and pro-tumorigenic macrophages from different human and murine cancer types. Data are pooled from at least two independent experiments. Each symbol represents one individual. All data are mean ± s.e.m and were analysed by two-tailed, unpaired Student’s t-test (a-d), one-tailed unpaired Student’s t-test (e). Box plot where whiskers represent the 5 and 95th percentile values, box limits represent the 25th and 75th percentiles, black line represents the median (e).