Figure 3. Coupling protocol and characterization of Trojan nano-horse.
(A) Table of the %co-ZnO values obtained during the parameters’ screening process; the best evidenced choices are in green. (B) Graphical schematization of the optimized coupling process. (C–E) Examples of optimized TNH Run 1 fluorescence colocalization images. (C) In purple, ZnO NCs labeled with Atto647, (D) in green, extracellular vesicles labeled with 3,3’-dioctadecyloxacarbocyanine perchlorate; (E) the TNH as colocalized in the merged channel, scale bar is 10 μm. Nanoparticle-tracking analysis measurements of (F) ZnO NCs, (G) extracellular vesicles and (H) TNHs in 1:1 v/v double distilled water and physiologic solution.
EV: Extracellular vesicle; PBS: Phosphate-buffered saline; RT: Room temperature; TNH: Trojan nano-horse; ZnO NC: Zinc oxide nanocrystal.