Fig. 6. TGFβ blocks cholesterol efflux gene induction after myelin uptake.
a, Schematic representation of the in vitro experimental approach to analyse induction of Abca1 and Abcg1 in microglia upon myelin treatment and TGFβ treatment. LPDS, lipoprotein-deficient serum; L929, L929 cell line conditioned media. b, Quantification of the average number of PLP+ myelin particles within phagocytes after 2 hours of incubation with myelin in TGFβ-treated and control cells. c,d, Quantification of the increase in mRNA expression of Abca1 and Abcg1 4hours (c) and 24hours (d) after myelin phagocytosis with or without TGFβ treatment in primary microglia (two-tailed Welch’s f-test). e, Quantification of the increase in mRNA expression of Abca1 and Abcg1 4hour after myelin phagocytosis with or without TGFβ treatment in BMDMs (two-tailed Welch’s f-test). f, Quantification of the increase in mRNA expression o’ Abca1 and Abcg1 induced by TGFβ in TGFβ-treated and control cells. No significant differences were observed (two-tailed Welch’s f-test). Solid lines in th graphs indicate the mean. P-values below 0.1 and n numbers are indicated in the figure.