Figure S5.
scATACseq Analysis, Related to Figure 5
(A) Two-dimensional UMAP representation of 13383 cells, based on genome-wide tile matrices of 500 bp bins, colored by pseudo-time values, as determined by trajectory analysis (left). The arrow indicates the approximated differentiation path. Gene activity scores of selected key genes of granulopoiesis (‘GMP-genes’: Irf8, Gata2, Cebpa; ‘pre-neutrophils / immature neutrophils-genes’: Gfi1, Runx1, Ltf; ‘mature neutrophils-genes’: Spi1, Klf2, Il1b, as previously defined (Evrard et al., 2018) are visualized as a function of the pseudo-time as dot plots (right).
(B) Violin plots showing gene activity scores of Ngp, Ltf, Camp, and Ifitm6 across the identified clusters.
(C) Violin plots showing gene activity scores of Il1b, C5ar1, Ifnar1, and Ifitm2 across the identified clusters.