Table 2.
Primers for real-time polymerase chain reaction (PCR) analysis of gene expression 1.
| Gene | PrimerBank ID | Forward Primer | Reverse Primer |
|---|---|---|---|
| Ucp1 | 6678497a1 | AGGCTTCCAGTACCATTAGGT | CTGAGTGAGGCAAAGCTGATTT |
| Pgc1α | 6679433a1 | TATGGAGTGACATAGAGTGTGCT | CCACTTCAATCCACCCAGAAAG |
| Ppar-α | 31543500a1 | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTGCAAAACCAAA |
| Ppar-γ | 6755138a1 | TCGCTGATGCACTGCCTATG | GAGAGGTCCACAGAGCTGATT |
| Fasn | 30911099a1 | GGAGGTGGTGATAGCCGGTAT | TGGGTAATCCATAGAGCCCAG |
| Atgl | 26327465a1 | GGATGGCGGCATTTCAGACA | CAAAGGGTTGGGTTGGTTCAG |
| Mgl | 6754690a1 | CGGACTTCCAAGTTTTTGTCAGA | GCAGCCACTAGGATGGAGATG |
| Hsl | 26325924a1 | CCAGCCTGAGGGCTTACTG | CTCCATTGACTGTGACATCTCG |
| Gapdh | 6679937a1 | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
1 The validated primers were obtained from Primerbank.