Figure 1.
Representative immunofluorescence images of CysLTR1 and Class III β-tubulin in polarized ARPE-19 cells. ARPE-19 cells were cultured for 7 days in low-serum medium. (a) CysLTR1 (visualized in red, indicated by white arrowheads), (b) class III β-tubulin (green) and (c) DAPI (blue) in polarized ARPE-19 cells. (d) Merged image of red, green and blue emission showing colocalization of CysLTR1 and class III β-tubulin (yellow, indicated by white arrowheads). Internalized CysLTR1 located near the cell nucleus was observed in individual cells, as indicated by the white arrow. e) Secondary antibody only control + DAPI (blue). Scale bar = 50 µm; n = 5.