Extended Data Table 3.
Processing of bulk sequencing BCR reads and number of nested PCR sequences and clonally distinct monoclonal antibody sequences
| Participant | Sample | Timepoint | Compartment | Cell count | Sequence count |
|||
|---|---|---|---|---|---|---|---|---|
| Input | Preprocessed | Post-QC | Unique VDJ | |||||
| 321-04 | 7 | d5 | Plasmablast | 101555 | 2433663 | 73442 | 70096 | 31219 |
| 321-04 | 10 | d0 | PBMC | 515000 | 2605448 | 99076 | 80997 | 33651 |
| 321-05 | 8 | d5 | Plasmablast | 8007 | 2495273 | 63677 | 50292 | 9898 |
| 321-05 | 11 | d0 | PBMC | 299000 | 2126862 | 75363 | 68028 | 33853 |
| 321-11 | 9 | d6 | Plasmablast | 2007 | 2771332 | 11526 | 8928 | 2076 |
| 321-11 | 12 | d0 | PBMC | 481000 | 2719946 | 114850 | 102198 | 83059 |
| Participant | Nested PCR
sequences |
QIV+ clonally
distinct mAb sequences |
||
|---|---|---|---|---|
| Plasmablast | Germinal center | Plasmablast | Germinal center | |
| 321-04 | 187 | 448 | 4 | 42 |
| 321-05 | 226 | 187 | 125 | 67 |
| 321-11 | 107 | 158 | 27 | 35 |