Figure 3: Plasma cytokine levels and release.
At all timepoints (baseline, day 3, day 7, and day 14) edetic acid plasma was collected and plasma IL-1β (A) and IL-6 (B) determined by Ella (Protein Simple). Data are shown as scatterplots with median for each group. Differences within each group between baseline and day 3 or day 7 were tested by Wilcoxon matched-pairs signed rank test. Plasma concentrations of dapansutrile at day 3 and day 7 for all individuals in the study were correlated to IL-1β (C) and IL-6 (D) release from stimulated peripheral blood mononuclear cells at the same timepoints. Spearman’s correlation was performed to analyse the correlation between the variables. Unstimulated peripheral blood mononuclear cellsfrom individuals taking 2000 mg/day and 100 mg/day were lysed and intracellular mature IL-1β and pro-IL-1β were measured by specific ELISA (E). Data are shown as mean ± SEM of relative change from baseline (value set at 1). One extreme outlier in each group was removed based on Grubb’s test. p values were calculated using Wilcoxon matched-pairs signed rank test for differences between day 0 and day 3. IL=interleukin.