Figure S2.
Binding Affinity and Neutralizing Abilities of VH3-53/VH3-66-Derived Antibodies, Related to Figure 6
(A) The distribution of IC50 against SARS-CoV-2 pseudovirus for VH3-53/VH3-66 derived antibodies revealed by high-throughput single-cell sequencing. Data for each antibody were obtained from a representative neutralization experiment, which contains three replicates. IC50 was calculated by using a four-parameter logistic curve-fitting and represented as mean. Each antibody’s heavy chain V-J gene is indicated on the x-axis, where the light chain V gene is indicated by different colors, as shown in the legend. A cross mark indicates that the antibody’s IC50 is higher than 1 μg/mL. The detailed characteristics of the antibodies shown here are listed in Table S2.
(B) Neutralization potency measured by a SARS-CoV-2 spike-pseudotyped VSV neutralization assay. Data for each NAb were obtained from a representative neutralization experiment, which contains three replicates. Data are represented as mean ± SD. IC50 and IC80 were calculated by fitting a four-parameter logistic curve.
(C) Measurement of the dissociation constant against RBD for the representing NAbs. All analyses were performed by using a serial 2-fold dilution of purified RBDs as the analyte, starting from 25 nM (magenta) to 1.56 nM (black).