Figure 4. cDC1 expression of CD40 is required for tumour rejection.
a, Tumour growth curves of (left) Xcr1+/+ CD40fl/fl and (right) Xcr1Cre/+ CD40fl/fl mice injected with 106 1956-mOVA.
b, Log2 expression of 148 genes with an increased expression of at least six-fold in skin draining LN (SDLN) cDC1 relative to splenic cDC1 (results averaged from biological triplicates).
c, Xcr1+/+ CD40fl/fl and Xcr1Cre/+ CD40fl/fl mice injected with 106 1956-EV or 106 1956-mOVA cells. Spleens stained for the presence of SIINFEKL-Kb tetramer+ CD8+ T cells. Representative flow plots of percentage of tetramer+ CD8 T cells. (Left) Representative flow plots of percentage of tetramer+ CD8 T cells. (Right) Graph of tetramer+ CD8 T cells as a percentage of all CD8 T cells. Data represent pooled biologically independent samples from two independent experiments (n=2 for Xcr1+/+ CD40fl/fl 1956-EV, n=4 for Xcr1+/+ CD40fl/fl, and n=6 for Xcr1Cre/+ CD40fl/fl). ** p=0.01 (unpaired, two-tailed Mann-Whitney test).
d, Graph of percent proliferated OT-I transferred into Xcr1+/+ CD40fl/fl, Xcr1Cre/+ CD40fl/fl immunized with cell-associated OVA on day 0. Data are pooled independent samples from two independent experiments (n=3 for Xcr1Cre/+ CD40fl/fl –OVA, n=4 for all other groups). *p=0.029 (unpaired, two-tailed Mann-Whitney test).
e, Graph of absolute number of proliferated OT-I per well after 72 h culture with ex vivo migratory cDC2 or cDC1 harvested from tumour-draining lymph nodes 1956-mOVA bearing mice. Data are pooled biologically independent samples from two independent experiments (n=3 for Xcr1+/+ CD40fl/fl Migratory cDC2, and n=4 for all other groups). *p=0.029 (unpaired, two-tailed Mann-Whitney test).
f, Graph of percent proliferated OT-II transferred into Xcr1+/+ CD40fl/fl, Xcr1Cre/+ CD40fl/fl immunized with cell-associated OVA on day 0. Data are pooled biologically independent samples from three independent experiments (n=4 for -OVA groups and n=7 for all other groups). ***p=0.006 (unpaired, two-tailed Mann-Whitney test).