Figure 4.
Ectopic ZDHHC1 inhibited EMT and disrupted stemness biomarkers in carcinoma cells. (A). Morphology changes of MCF7 and HONE1 cells observed under phase contrast microscopy. (B). Immunofluorescent staining of epithelial markers (E-cadherin, Occludin) and mesenchymal markers (N-cadherin, Vimentin) in MCF7 cells transfected with ZDHHC1 or Vector. (C). Western blot assays of epithelial markers (E-cadherin, Occludin) and mesenchymal markers (N-cadherin, Vimentin) in MCF7 and HONE1 cells transfected with ZDHHC1 or Vector. β-actin was taken as internal control. (D). RT-PCR assays of cancer stemness markers (NANOG, SOX2, OCT4, CD44, ABCG2 and BMI1) in MCF7 and HONE1 cells, with ZDHHC1 or empty vector transfected. β-actin was taken as control. (E-F). Spheroid formation assay showed that ZDHHC1 decreased the size of spheres formed by MCF7 and HONE1 cells. ***p < 0.001.