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Melting temperatures of WT and mutant AmpC enzymes
| Enzyme | Avg Tm (°C) ± SDa | Avg ΔTm (°C) ± SEb |
|---|---|---|
| WT | 55.33 ± 0.06 | 0.00 ± 0.080 |
| E247K | 45.80 ± 0.00 | −9.53 ± 0.060 |
| G183D | 50.43 ± 0.15 | −4.90 ± 0.200 |
| T96I | 49.23 ± 0.06 | −6.10 ± 0.080 |
| ΔG229–E247 | 51.77 ± 0.06 | −3.56 ± 0.080 |
Values represent averages from three technical replicates.
Changes in Tm relative to that of WT AmpC.
