Fig. 4.
(A) Multiplex immunohistochemical analysis of a GBM showing p-STAT3 expression (green) in CD3 T cells (blue) as they exit from the tumor vasculature that is lined with CD163 M2 macrophages (red). Glial fibrillary acidic protein (GFAP)+ glioma cells are shown in orange, and the nuclei are counterstained with 4′,6′-diamidino-2-phenylindole in blue (100 µm scale bar). (B) M2 macrophage (denoted in red) is directly interacting with a CD3 T cell (blue) that has p-STAT3 in its nucleus within the GBM microenvironment (50 µm scale bar).