Fig. 4. Exportin-1 plays a role in the subcellular localization of polκ.
(A) Immunofluorescence staining of A375 cells treated with DMSO or 5 μM PLX4032 ± 20 μM leptomycin B (LMB) for 6 hours. Scale bar, 10 μm. (B) Quantification of the percentage of cells with nuclear enrichment of polκ after 3 or 6 hours. Mean ± S.E.M; at least 100 cells were counted for each sample across 4–8 fields of view. **P < 0.01, ***P < 0.001, paired two-tailed t-test. (C) Schema detailing the experiment design for the leptomycin B (LMB) recovery assay subsequently shown in (D and E). (D) Immunofluorescence staining of A375 cells treated with DMSO or 5 μM PLX4032 for 24 hours and then DMSO or 10 μM LMB for 24 hours. Scale bar, 10 μm. (E) Quantification of the percentage of cells with nuclear enrichment of polκ. Mean ± S.E.M; at least 100 cells were counted for each sample across 7 fields of view. *P < 0.05, **P < 0.01, ***P < 0.001, paired two-tailed t-test.