Figure 4. Circadian timing effects the expression of genes relating to reward (a), thirst and hunger (b).

The expression of Drd2, Oprml and Ghrl were significantly elevated during the dark cycle compared to the light cycle. By contrast, the expression of Avp, was significantly elevated during the light compared to the dark cycle. The expression of Drd1, Th, Lepr and Rxfpl was unaffected by light-cycle. All data was analysed using a paired two-tail t-test. Summary values represented as mean±SEM; n=3, *p < 0.05; **p < 0.01.