Fig. 5.
AhR binds and transactivates the Prkn promoter gene. A) ChIP analysis of AhR binding to the mouse Prkn gene promoter region. Material for immunoprecipitation was obtained from the brain tissues of mice treated with corn oil as vehicle (V, lane 3) or a single oral dose of TCDD (T, lane 4). PCR products corresponding to the −407/+74 bp region of the Prkn gene promoter were generated. Genomic DNA was used as the positive control (C, lane 5) and IgG antibody was used as the negative control (IgG, lane 2). MW (lane 1) represents molecular weight markers. A representative gel of two independent experiments is shown. B) The luciferase activity of SH-SY5Y cells (1 × 106) transfected with 1, 2.5, 5 or 10 μg of the pGL4/−419_mPrkn vector containing the firefly luciferase open reading frame under the control of the mouse Prkn gene promoter and treated with 10 nM of TCDD or DMSO (vehicle) for 48 h was determined. The luciferase activity was normalized to Renilla luciferase activity and is expressed as the mean ± S.D. of three independent experiments. * p < 0.05, control vs. treatment.