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. Author manuscript; available in PMC: 2020 Aug 8.
Published in final edited form as: Cell. 2019 Aug 8;178(4):795–806.e12. doi: 10.1016/j.cell.2019.07.008

Figure. 3.

Figure. 3.

Commensal microbiome from LTS PDAC patients induces a strong immune infiltration and antitumoral immune response.

(A) Immunohistochemical (IHC) staining of CD3, CD8 and Granzyme B from tumors of STS and LTS PDAC patients (representative picture).

(B) Quantification of IHC of CD3, CD8 and Granzyme B on STS and LTS PDAC patients.

(C) Representive pictures of multiplex immunofluorescence staining (Multiplex IF) with Opal kit.

(D) Immunohistochemical staining of CD8 from tumors of STS and LTS PDAC patients from validation cohorts (JHH) (representative picture).

(E) Quantification of IHC of CD8 on STS and LTS PDAC patients from validation cohorts (JHH).

(F) Spearman correlation between CD3+, CD8+ and GzmB+ tissue densities and the overall survival (upper panel) and alpha diversity by Shannon Index (lower panel) of all PDAC patients.

(G) Spearman correlation between CD8+ tissue densities and Saccharopolyspora, Pseudoxanthomonas and Streptomyces (p < 0.0001, p = 0.006 and p < 0.0001, respectivelly) in PDAC patients.