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. 2020 May 26;58(6):e00557-20. doi: 10.1128/JCM.00557-20

TABLE 3.

Relative sensitivities of SARS-CoV-2 detection assays using 4 different primer-probe sets and the BGI testing kit

Primer-probe set or kit Dilution Viral copiesa Sample ID No. of replicates
 % positivity Mean CTb ,c SD
Tested Positive Negative
Corman E-gene 1:105 63 S5 20 20 0 100 33.7 1.13
1:2 × 105 31.5 S5_2 20 20 0 100 34.7 0.69
1:5 × 105 12.6 S5_5 20 16 4 80 36.6 1.60
1:106 6.3 S6 20 17 3 85 37.2 1.34
1:107 0.63 S7 20 3 17 15 37.9 0.10
Corman RdRp gene 1:104 630 S4 20 20 0 100 35.2 1.34
1:2 × 104 315 S4_2 20 16 4 80 36.8 1.26
1:105 63 S5 20 17 3 85 36.6 1.84
1:106 6.3 S6 20 2 18 10 35.8 1.79
1:107 0.63 S7 20 1 19 5 37.5 0.00
CDC N1 1:105 63 S5 20 20 0 100 33.7 1.45
1:2 × 105 31.5 S5_2 20 17 3 85 35.8 1.82
1:5 × 105 12.6 S5_5 20 12 8 60 36.5 1.58
1:106 6.3 S6 20 13 7 65 36.2 1.41
1:107 0.63 S7 20 2 18 10 36.2 0.55
CDC N2 1:105 63 S5 20 20 0 100 33.1 1.26
1:2 × 105 31.5 S5_2 20 20 0 100 35.1 0.98
1:5 × 105 12.6 S5_5 20 17 3 85 36.4 1.23
1:106 6.3 S6 20 18 2 90 36.8 1.35
1:107 0.63 S7 20 3 17 15 37.2 0.61
BGI kit 1:105 63 S5 20 20 0 100 31.6 2.39
1:2 × 105 31.5 S5_2 20 20 0 100 33.8 1.31
1:5 × 105 12.6 S5_5 20 18 2 90 36.2 1.94
1:106 6.3 S6 20 10 10 50 34.7 2.06
1:107 0.63 S7 20 0 20 0
a

Number of viral copies (genomic equivalents) per reaction.

b

Cycle threshold.

c

Only positive results are included in calculation of mean CT.