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. 2020 Apr 14;39(21):4271–4285. doi: 10.1038/s41388-020-1289-1

Fig. 2. L1CAM expression inversely correlates with CSC content and function.

Fig. 2

a qPCR analysis for L1 and CSCs genes in L1 sorted cells. Data are normalised to GAPDH and are presented as fold change in gene expression relative to L1high cells. *p < 0.05; **p < 0.005; ***p < 0.0005. n ≥ 6. b Sphere formation capacity of L1 sorted cells. 200 cells per well. **p < 0.005 compared with L1low. n ≥ 6. c Formation of organoid-like structures of L1 sorted cells. **p < 0.005 compared with L1low. n ≥ 6. d Representative images of organoid-like structures derived from L1 sorted cells. e qPCR analysis for EMT genes in organoid-like structures derived from L1 sorted cells. Data are normalised to GAPDH and are presented as fold change in gene expression relative to L1high cells. *p < 0.05; **p < 0.005; ***p < 0.0005. n ≥ 6. f Migratory potential of L1 sorted cells. ***p < 0.0005 compared to L1low. n ≥ 3. g Growth capacity of L1 sorted cells in presence of 100 μM of Gemcitabine (GEM). **p < 0.005 compared with L1low. n ≥ 6. h–i Kaplan–Meier curve of L1 sorted cells subcutaneously xenografted into athymic mice. Long-rank (Mantel–Cox) test ***p < 0.0005 compared with L1low. n = 8. j Representative histological sections of xenografts derived from L1 sorted cells. Tumour sections were (immuno)stained for Haematoxylin & Eosin (H&E), CD31, CALD1, E-CADHERIN (CDH1), KERATIN 17. S stroma, T tumour. k Quantification of fibrotic area on H&E stained sections and of CD31, CALD1, CDH1, F4/80 and KERATIN 17 detected by IHC. *p < 0.05, compared with L1low.