KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Monoclonal anti- β-actin | Cell Signaling | Cat#4970; RRID: AB_2223172 |
| Rabbit polyclonal anti-phospho-Akt (Ser473) | Cell Signaling | Cat#9271; RRID: AB_329825 |
| Rabbit polyclonal anti-Akt | Cell Signaling | Cat#9272; RRID: AB_329827 |
| Rabbit Monoclonal anti-phospho-mTOR (Ser2448) | Cell Signaling | Cat#5536; RRID: AB_10691552 |
| Rabbit Polyclonal anti-mTOR | Cell Signaling | Cat#2972; RRID: AB_330978 |
| Rabbit Polyclonal anti-Phospho-S6 (S240/244) | Cell Signaling | Cat#2215; RRID: AB_331682 |
| Rabbit Monoclonal anti-S6 Ribosomal Protein | Cell Signaling | Cat#2217; RRID: AB_331355 |
| Rabbit Monoclonal anti-Phospho-4EBP1 | Cell Signaling | Cat#;2855 RRID: AB_560835 |
| Mouse Monoclonal anti-4EBP1 | Santa Cruz | Cat#sc-9977; RRID: AB_626621 |
| Rabbit Monoclonal anti-Phospho-p70 S6 Kinase (Thr389) |
Cell Signaling | Cat#9234; RRID: AB_2269803 |
| Rabbit Monoclonal anti-p70 S6 Kinase | Cell Signaling | Cat#2708; RRID: AB_390722 |
| Rabbit Monoclonal anti-Phospho-ULK1 (S757) | Cell Signaling | Cat#14202; RRID: AB_2665508 |
| Rabbit Monoclonal anti-Rictor | Cell Signaling | Cat#2114; RRID: AB_2179963 |
| Rabbit Polyclonal Anti-Acetyl-Histone H4 (Lys16) | Millipore Sigma | Cat#07-329; RRID: AB_310525 |
| Rabbit Polyclonal anti-AMPKα | Cell Signaling | Cat#2532; RRID: AB_330331 |
| Rabbit Monoclonal anti-Phospho-AMPKα (Thr172) | Cell Signaling | Cat#2535; RRID: AB_331250 |
| Goat Anti-Mouse IgG (H+L)HRP Conjugate | Bio-Rad | Cat#1721011; RRID: AB_11125936 |
| Goat Anti-Rabbit IgG (H+L)HRP Conjugate | Bio-Rad | Cat#1706515; RRID: AB_11125142 |
| Mouse Monoclonal anti-ATP synthase beta subunit | Abcam | Cat# Ab14730; RRID: AB_301438 |
| Mouse Monoclonal anti-Oct3/4 | Santa Cruz | Cat#sc-5279; RRID: AB_628051 |
| Mouse Monoclonal anti-Lkb1 | Santa Cruz | Cat#sc-32245; RRID: AB_627890 |
| Alexa Fluor 488 Goat-Anti-Mouse | Thermo Fisher Scientific | Cat#A11029; RRID: AB_138404 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Mouse LIF Protein | Millipore Sigma | Cat#ESG1107 |
| Gelatin from Porcine Skin | Sigma-Aldrich | Cat#G1890 |
| Matrigel | Corning | Cat#356231 |
| Agarose Low Melting Point | RPI | Cat#9012-36-6 |
| Fetal Bovine Serum | BioWest | Cat#S1620 |
| PD0325901 (MEK inhibitor) | Selleckchem | Cat#S1036 |
| Y-27632 2HCl (ROCK inhibitor) | Selleckchem | Cat#S1049 |
| CHIR-99021 (GSK3 inhibitor) | Selleckchem | Cat#S1263 |
| Sapanisertib (INK-128) (mTORC1/2 inhibitor) | Medchem | Cat#HY-13328 |
| Hoechst 33342 Solution | Sigma-Aldrich | Cat#62249 |
| 2-Deoxy-D-glucose | Sigma-Aldrich | Cat#D8375 |
| L-γ-Glutamyl-p-nitroanilide (GPNA) (Glutamine Transporter inhibitor) | MP Biomedicals | Cat#67953-08-6 |
| AICAR (AMPK activator) | Sigma-Aldrich | Cat#A9978 |
| Compound C (AMPK inhibitor) | Sigma-Aldrich | Cat#171260 |
| Lipofectamine RNAiMAX | Thermo Fisher Scientific | Cat#13778075 |
| OPTIMEM Reduced Serum Medium | Thermo Fisher Scientific | Cat#31985 |
| DMEM, high glucose, GlutaMAX Supplement | Thermo Fisher Scientific | Cat#10566 |
| DMEM, no glucose | Thermo Fisher Scientific | Cat#11966 |
| MEM Non-Essential Amino Acids (NEAA) | Thermo Fisher Scientific | Cat#11140 |
| Sodium Pyruvate | Thermo Fisher Scientific | Cat#11360 |
| Trypsin-EDTA (0.05%), phenol red | Thermo Fisher Scientific | Cat#25300 |
| 2-Mercaptoethanol | Sigma-Aldrich | Cat#M7522 |
| Penicillin-Streptomycin | Thermo Fisher Scientific | Cat#15070 |
| Experimental Models: Cell Lines | ||
| Mouse ESC R1 (LL) | Nagy lab | Nagy et al., 1993 |
| Mouse ESC R1 (SL) | this manuscript | N/A |
| Mouse ESC R1 (SS) | this manuscript | N/A |
| Experimental Models: Organisms/Strains | ||
| C57BL/6 | Jackson Laboratories | Cat#000664 |
| C57BL/6 | Charles River Laboratories | Cat#027 |
| B6C3 | Jackson Laboratories | Cat#100010 |
| B6D2 | Jackson Laboratories | Cat#100006 |
| Software and Algorithms | ||
| R | The R Foundation | https://www.r-project.org/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Microsoft Excel | Microsoft | https://products.office.com/en-us/excel |
| Microsoft PowerPoint | Microsoft | https://products.office.com/en-us/powerpoint |
| Prism GraphPad Software | Prism | https://www.graphpad.com/scientificsoftware/prism/ |
| Biorender App | Biorender | https://app.biorender.com |