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. 2020 May 1:eabc1669. doi: 10.1126/science.abc1669

Fig. 1. SARS-CoV and SARS-CoV-2 infect 2D human airway cultures.

Fig. 1

(a) Live virus titers can be observed by virus titrations on VeroE6 cells of apical washes at 2, 24, 48, 72 and 96h after infection with SARS-CoV (blue) and SARS-CoV-2 (red). The dotted line indicates the lower limit of detection. Error bars represent SEM. N=4. *P<0.05, **P<0.01, ***P<0.001. (b and c) Immunofluorescent staining of SARS-CoV-2 (b) and SARS-CoV (c) infected differentiated airway cultures. Nucleoprotein (NP) stains viral nucleocapsid (red), which colocalized with the ciliated cell marker AcTUB (green). Goblet cells are identified by MUC5AC (blue). Nuclei are stained with TO-PRO3 (white). Scale bars indicate 20μM. Top panels are side-view while bottom panels are top-view.