Table 6.
Detection of ISAV RNA segment 8 by conventional RT–PCR and SYBR Green real-time RT–PCR in different tissues of fish at 76 days post-ISAV challenge
| Method | Fish species | Fish tissues |
|||||
| Liver | Spleen | Kidney | Heart | Caeca | Gill | ||
| Conventional RT–PCR | Atlantic salmon | +a | −b | + | + | − | + |
| Rainbow trout | + | + | − | − | + | − | |
| Real-time RT–PCR | Atlantic salmon | + (36.44,c 0.0022d) | + (37.92, 0.001) | + (37.71, 0.001) | + (35.61, 0.0036) | + (35.99, 0.0029) | + (39.71, NCe) |
| Rainbow trout | + (35.75, 0.0033) | + (37.98, 0.001) | − | + (36.61, 0.0020) | + (36.43, 0.0022) | + (38.04, NC) | |
a
+ Denotes positive by conventional one-tube RT–PCR or real-time RT–PCR detection format.
b
− Denotes negative by either agarose gel electrophoresis for conventional one-tube RT–PCR or real-time RT–PCR detection format.
c
Ct value.
d
ISAV RNA concentration (ng/μl) extrapolated from the standard curve
e
NC denotes not calculated.