Figure 7.
Macrophages were pre-activated (2 hours) with LPS (100 ng/ml, A and B), and then treatments were added overnight. IL-6 (A) and TNFα (B) were measured in the supernatant by enzyme linked immunosorbent assay (ELISA). C shows the quantification of reduced fluorescence of Amplex Red after 5 hours incubation with treatments and 150 SM H2O2 (without cells), measured 1h after assay stabilization. Data is presented as mean of triplicates assayed three times with similar results ± SD; * p < 0.05, *** p < 0.001