Figure 6.
CP ILC2 possess more potent proliferative and cytokine-producing capability than meningeal ILC2 in aged mice. (A) Number of ILC2 at different regions of aged mice after daily IL-33 treatment for 7 d. Data are from four mice per group and are representative of two independent experiments. CTX, cortex. (B) ILC2 from the meninges of young mice and the meninges of aged mice and CP from aged mice were sorted and cultured with IL-7, IL-2, IL-33, and SCF for up to 4 wk. Fold change of cell numbers over time is shown. Data are from four mice per group and are representative of two independent experiments. (C) Numbers of meningeal and CP ILC2 in aged mice treated with PBS or IL-33 daily for 7 d. Data are from four mice per group and are representative of two independent experiments. (D) Fold change of ILC2 numbers in IL-33–treated mice over those in PBS-treated mice. The average ILC2 number in PBS-treated mice was set as 1. Data are from four mice per group and are representative of two independent experiments. (E) Representative flow cytometry profiles of IL-5 and IL-13 expression in meningeal and CP ILC2 in IL-33–treated aged mice. (F) MFI of IL-5 and IL-13 in meningeal and CP ILC2 in IL-33–treated aged mice. Data are from five mice per group and are representative of two independent experiments. (G) scRNA-Seq was performed with sorted meningeal and CP ILC2 from IL-33–treated aged mice. UMAP analysis of the combined ILC2 and meningeal ILC2 population. (H) Expression of the indicated genes by different subsets of ILC2 in the combined meningeal and CP ILC2 population. (I) Split analysis of meningeal and CP ILC2 by UMAP. (J) Percentage of each ILC2 subset in meningeal and CP ILC2. (K) Expression of the indicated genes by meningeal and CP ILC2. (L) Expression of the indicated genes in each ILC2 subset in meningeal and CP ILC2. Error bars are mean ± SEM. *, P < 0.05; **, P < 0.01.