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. 2005 May 31;333(1):186–193. doi: 10.1016/j.bbrc.2005.05.089

Fig. 7.

Fig. 7

Epitope mapping of scFv B1 by Western blot analysis. (A) scFv B1 recognizes S (12–1194)-Fc protein but does not bind to S1 (12–672)-Fc protein; (B) the proteins S (12–1194)-Fc and S1 (12–672)-Fc are recognized by anti-human Fc antibody as control; (C) SDS–PAGE analyzes the expression of S2 protein and its fragments; (D) Western blot shows the binding of scFv B1 to S2 (680–1189) and S2 (1023–1189), but does not recognize the S2(680–1029) and S2 (680–879).