Figure 2.
IL-10-deficient mice show severe lung pathology after A. baumannii infection. WT and IL-10-deficient mice were infected i.n. with A. baumannii (3 × 107 CFU/30 μl) and euthanized at 1 day and 3 days after the infection. (A,B) Lung histopathology was evaluated in H&E-stained sections and scored as described in the Materials and Methods. Bar = 100 μm (C) MPO activities in lung homogenates of WT and IL-10-deficient mice were measured. (D) The total number of BAL fluid cells was determined with a hemocytometer. (E,F) Differential counts of macrophages and neutrophils were obtained by Diff-Quik staining. (B–F) Results are combined from two independent experiments and presented as means ± SD. *P < 0.05 and ***P < 0.001.