Figure 3.
(A) The scratch width was measured and photographed at the beginning of the scratch test and 36 h later. Relative width of scratch was detected (B) at the beginning of the scratch test and (C) 36 h later. (D/E) Transwell assay was carried out to evaluate the metastatic potential of SKOV-3 cells after treated with TPD@TB/KBU2046 (10 μg/ml), PD@TB/KBU2046 (10 μg/ml), TPD@TB (10 μg/ml), TPD@KBU2046 (10 μg/ml) and TPD (10 μg/ml), respectively. The results were represented as mean ± SD (n =3). The data were analyzed via two-sided Student's t-test. *** P <0.001. (F) After 36 treated with TPD@TB/KBU2046 (10 μg/ml), PD@TB/KBU2046 (10 μg/ml), TPD@TB (10 μg/ml), TPD@KBU2046 (10 μg/ml) and TPD (10 μg/ml) for 36 h, the expression level of Vimentin in SKOV-3 cells was determined.