Figure 5.
Representative microphotographs of IBA1 and PGRN immunostained tissue in the region of the SNpc (A) and PFC (B) of a healthy control and PD patient. Presence of IBA1+ microglia significantly increased in the nigra of PD compared to healthy control, yet despite the abundant quantity of microglia, few PGRN-immunoreactive cells resemble microglia morphology (arrows) in the SNpc (A). Instead, PGRN is detected in neuromelanin cells or present as neuronal cell bodies (arrow heads) and in perivasculature spaces (blue asterisk). IBA1-immunoreactivity is similar in the PFC between healthy control and PD patient, and PGRN+ cells largely resemble microglial morphology (arrows) in both subjects (B). Scale bar 100 μm, inset 50 μm.