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. Author manuscript; available in PMC: 2021 Jan 22.
Published in final edited form as: Neuron. 2019 Nov 20;105(2):260–275.e6. doi: 10.1016/j.neuron.2019.10.010

Figure 4: α-syn is pooled and phosphorylated in dystrophic neurites surrounding Aβ plaques.

Figure 4:

A) Immunoblots were conducted on whole brain lysates from 10 mo old non-injected WT and 5xFAD mice as shown in the figure and described in results. Quantification showed similar levels of endogenous mouse α-syn (B) and tau (C) proteins. Scale bar, 40 μm. D) As shown here and described in results, IF triple-labelling showed the re-localization and pooling of endogenous α-syn (red) APP positive neurites (green) around Aβ plaques (grey), using 9027, 22C11, and NAB228, respectively. (E) IF triple-labelling of p-α-syn (red) in dystrophic neurites (green) around Aβ plaques (grey) was conducted using MJFR13, 22C11, and NAB228, respectively. White scale bar: 20 μm. A two-tailed t-test was performed. Data are presented as mean ± s.e.m. (n=5 mice per group).