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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Nat Protoc. 2019 Sep 23;14(10):2986–3012. doi: 10.1038/s41596-019-0210-2
Component Volume per reaction Volume for four replicates
Ultragrade water 12.32 μL 61.6 μL
HEPES pH 6.8, 1M 0.4 μL 2 μL
MgCl2, 1M 0.18 μL 0.9 μL
rNTP solution mix, 25 mM each 0.8 μL 4 μL
LwaCas13 in SB (63.3 μg/mL) 2 μL 10 μL
Murine RNase Inhibitors, 40 U/μL 1 μL 5 μL
T7 RNA polymerase, 5 U/μL 0.5 μL 2.5 μL
crRNA (10 ng/μL) 1 μL 5 μL
LF-RNA sensor 1 (100 μM) 0.2 μL 1 μL
Total 19 μL 95 μL