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. 2020 Jan 2;30:101419. doi: 10.1016/j.redox.2019.101419

Fig. 7.

Fig. 7

WWP2 inhibits the endothelial injury complex Septin4-PARP1 and alleviates endothelial cells injury. (A) HUVECs underwent transfection with negative control or WWP2 siRNAs for 36 h and then an interaction between PARP1 and Septin4 was assessed by co-immunoprecipitation. (B) HUVECs underwent transfection with control-HA-tagged or HA-WWP2, Myc-PARP1 and Flag-Septin4 for 36 h. The interaction between PARP1 and Septin4 was then assessed by co-immunoprecipitation. (C) HUVECs underwent transfection with Flag-control or Flag-WWP2 for 36 h and then treated with 500 μmol/L H2O2 for 12 h. Cleaved PARP1, cleaved caspase-3, and Septin4 were detected by western blotting. (D) Quantitated data are shown as means ± SD (***P < 0.001, unpaired Student's t-test; ##P < 0.01, #P < 0.05, two-way ANOVA with Bonferroni's post-hoc test). (E) HUVECs underwent transfection with negative control or WWP2 siRNAs for 36 h and were then treated with 500 μmol/L H2O2 for 12 h. Cleaved PARP1, cleaved caspase-3, and Septin4 were detected by western blotting. (F) Quantitated data are shown as means ± SD (***P < 0.001, unpaired Student's t-test; ##P < 0.01, #P < 0.05, two-way ANOVA with Bonferroni's post-hoc test).