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. 2020 Jan 6;11(1):9. doi: 10.1038/s41419-019-2206-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Western blot of Wnt7a in Sham, Stroke + PBS, and Stroke + OPC groups. b Western blot revealed Wnt7a increased in Stroke + OPC group after ischemia compared with Stroke + PBS group. Data are mean ± SD, n = 3 per group, *p < 0.05, as compared with Stroke + PBS group, ^#p < 0.05, as compared with Sham group. S + P: Stroke + PBS; S + O: Stroke + OPC. c Images showed the β-catenin (green) expression of bEnd.3 cells in control (CON), CM of OPC, Wnt7a, and CM of OPC + SI groups at 24 h after OGD. Statistical analysis indicated CM of OPC and Wnt7a could promote β-catenin expression in bEnd.3 cells compared with control. Data are mean ± SD, n = 4 per group, **p < 0.01, as compared with CON group, ^#p < 0.05, ^##p < 0.01, as compared with CM of OPC + SI group. d Images showed the claudin-5 (green) expression of bEnd.3 cells in CON, CM of OPC, Wnt7a, and CM of OPC + SI groups at 24 h after OGD. Data are mean ± SD, n = 4 per group, *p < 0.05, **p < 0.01, as compared with CON group, ^#p < 0.05, ^##p < 0.01, as compared with CM of OPC + SI group. e Experimental setup for Evans blue determination in vitro. f Bar graph indicated the relative permeability of endothelial barrier in vitro. Data are mean ± SD, n = 4 per group, ***p < 0.001, as compared with CON group, ^###p < 0.001, as compared with CM of OPC + SI group. Scale bar = 50 µm.