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DNA damage induced by 177Lu-PSMA-617 RLT using high, intermediate, or low specific activity (experiment 1). (A) Representative areas of tumor tissue stained for γ-H2A.X are shown at 10-fold magnification (conditions in separate rows). Proportion of γ-H2A.X–positive cells was evaluated by a masked reader visually (A) and by ImageJ software (B). + = 5%–10%; ++ = 10%–20%; +++ = >20% γ-H2A.X–positive cells. Dots represent single values; bars are mean values (n = 3 per group).
