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. 2019 Dec 13;20(24):6309. doi: 10.3390/ijms20246309

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The PERK-eIF2α signaling pathway induces GADD45α expression. (A) Heatmap of the gene ontology (GO) enriched genes belong to the term “regulation of cell cycle” (GO:0051726). (B) Cell lysates were obtained from wild type (Eif2α^S/S) or eIF2αSer51Ala mutant (Eif2α^A/A) MEFs at the indicated time points following treatment with DMSO or thapsigargin (TG; 300 nM) (n = 3). α-Tubulin was used as an endogenous control. (C) Total RNAs were obtained from Eif2α^S/S or Eif2α^A/A MEFs at the indicated time points following treatment with DMSO or TG (300 nM) (n = 3). Gapdh primers were used as an endogenous control. (D,E) Total RNAs were obtained from wild type MEFs at 8 h following treatment with DMSO or TG (300 nM) in the presence or absence of 4μ8C (1 μM) (D) or ceapinA7 (0.5 μM) (E) (n = 3). Gapdh primers were used as an endogenous control. ** p < 0.01, *** p < 0.005.