Figure 3.
P47G promotes and W60A abrogates homodimer formation. A, C1R-B*27:05, C1R-B*27:03, C1R-B*27:03-P47G, and C1R-B*27:05-W60A cell lysates were separated by 12% SDS-PAGE and analyzed by immunoblotting using the HC10 antibody and an antibody recognizing GAPDH as loading control. Representative blots are shown. The addition of H2O2 to the cells is indicated above the lanes. Red arrow, HLA-B27 homodimers; blue arrow, monomeric forms. B, background-corrected densitometric analyses showing the ratio of HLA-B27 homodimer to monomer. n = 3, mean ± S.E. (error bars). p value was calculated using the Welch's t test (*, p < 0.05; **, p < 0.001; NS, nonsignificant).