Table 2.
Effects of TGFβ1, TGFβ2 and miR-29a mimic on gene expression in cardiac fibroblast cultures.
| Genes | TGFβ1 | TGFβ1 + miR-29a mimic | TGFβ1 + control mimic | TGFβ2 | TGFβ2 + miR-29a mimic | TGFβ2 + control mimic | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FC | P vs. con | FC | P vs. TGFβ1 | FC | P vs. con | FC | P vs. con | FC | P vs. TGFβ2 | FC | P vs. con | |
| TGFBR1 | 1.1 | 0.12 | 1.1 | 0.7 | 1.1 | 0.1 | 0.8 | 0.4 | 0.7 | 0.2 | 0.7 | 0.1 |
| TGFBR2 | 1.1 | 0.3 | 1.0 | 0.3 | 1.2 | 0.2 | 0.8 | 0.2 | 0.6 | 0.3 | 0.7 | 0.07 |
| CTGF | 2.3 | 0.002 | 1.8 | 0.09 | 4.8 | 0.02 | 6.5 | 3.2E-05 | 2.2 | 0.002 | 4.1 | 0.01 |
| miR-29a | 0.4 | 0.02 | NA | NA | 0.4 | 0.02 | 0.5 | 0.03 | NA | NA | 0.5 | 0.006 |
| COL1A1 | 1.6 | 0.01 | 0.1 | 0.004 | 1.9 | 0.003 | 2.4 | 0.01 | 0.2 | 0.005 | 2.8 | 0.003 |
| COL1A2 | 2.0 | 0.03 | 0.2 | 0.01 | 2.1 | 0.001 | 1.7 | 0.01 | 0.1 | 0.0007 | 1.6 | 0.02 |
| COL3A1 | 1.5 | 0.02 | 0.1 | 0.004 | 1.3 | 0.01 | 2.0 | 0.01 | 0.3 | 0.0007 | 2.0 | 0.009 |
| ELN | 1.9 | 0.01 | 0.3 | 0.003 | 3.2 | 0.02 | 2.6 | 0.01 | 0.1 | 0.01 | 3.0 | 0.01 |
Expression of miR-29a, select pro-fibrotic genes in cardiac fibroblasts, following treatment with TGFβ1 (2 ng/mL) or TGFβ2 (1 ng/mL) with/without miR-29a mimic. Data are expressed as linear fold change (FC); n = 3 for each condition; all experiments were repeated 3 times. One-way ANOVA and Tukey's test were used to compare TGFβ1/2, TGFβ1/2 + miR29a-mimic, TGFβ1/2 + control-mimic and control. TGFBR1, Transforming growth factor, beta receptor 1; TGFBR2, Transforming growth factor, beta receptor 2; CTGF, Connective tissue growth factor; miR-29a, microRNA 29a; COL1A1, Collagen type I alpha 1 chain; COL1A2, Collagen type I alpha 2 chain; ELN, Elastin.