TABLE 3.
Plasmids used in this study
| Plasmid | Descriptiona | Reference or source |
|---|---|---|
| pRK2013 | oricolE1, RK2 derivative, Kanr, mob+ tra+ | 62 |
| pGPI-SceI | oriR6K, I-SceI recognition sequence, Tmpr | 45 |
| pDAI-SceI | DHFR promoter controlling e-I-SceI, Tetr | 45 |
| pBAD30 | orip15A araC PBAD Ampr | 63 |
| pTL5 | pBAD30 containing CepR (BCAM1868) with Shine-Dalgarno region | This study |
| pBBRlux | luxCDABE-based promoter plasmid | 64 |
| pTL7 | pBBRlux containing cepI promoter region (upstream of BCAM1870) | This study |
| pTL12 | pBBRlux containing araC and PBAD promoter | This study |
| pTL13 | pBBRlux containing dhfr promoter region | This study |
| pTL17 | pGPI-SceI containing custom gene fragment for the deletion of paaK1 (BCAL0404) in K56-2 | This study |
| pTL18 | pGPI-SceI containing custom gene fragment for the deletion of paaK2 (BCAM1711) in K56-2 | This study |
| pTnMod-STp | oripSC101, Tn5 inverted repeats, Tn5 transposase, Tmpr | 65 |
| pTL9 | BglII restriction digest of pTnMod-STp to remove Tn5 transposase | This study |
| pTL21 | oripSC101 rhaR rhaS PrhaB, Tmpr | This study |
| pTL22 | pTL21 expressing rhamnose-inducible paaK (ECW_m1532) | This study |
| pAAD | 15.4-kb region contains paa cluster from E. coli W | 31 |
| pSCrhaB2 | oripBBR1 rhaR rhaS PrhaB mob+, Tmpr | 48 |
| pKF2 | pSCrhaB2 expressing rhamnose-inducible paaK2 (BCAM1711) | This study |
| pCP300 | cepI::luxCDABE transcriptional promoter fusion in pMS402 | 47 |
| pPromcepR | cepR::luxCDABE transcriptional promoter fusion in pMS402 | 47 |
| pMS402 | luxCDABE-based promoter reporter plasmid, Kanr Tpr | 47 |
| pDelCepR | pGPI-SceI containing the upstream and downstream regions of cepR for markerless deletion in K56-2 | 47 |
| pDelCepI | pGPI-SceI containing the upstream and downstream regions of cepI for markerless deletion in K56-2 | 47 |
a
Ampr, ampicillin resistance; Kanr, kanamycin resistance; Tmpr, trimethoprim resistance; Tetr, tetracycline resistance.