Figure 3.
PRC2 Restricts the Interferon-Induced Activation of MHC-I APP Genes in Multiple MHC-I Low Tumor Types
(A) Cell surface MHC-I in K-562 cells expressing H3.3 WT or H3.3 K27M treated with IFN-γ 10 ng/mL for 24 h.
(B) Immunoblot in neuroblastoma (Kelly) cells treated with EPZ-011989 3 μM for 10 days ± IFN-γ 10 ng/mL for 24 h.
(C and D) Cell surface MHC-I following incubation with IFN-γ 10 ng/mL for 24 h in indicated cell lines pretreated with EPZ-011989 3 μM (C) and K-562 cells pretreated with EED-226 (D). Analysis after 10 days of inhibitor treatment.
(E) Cell surface MHC-I in EED KO or parental control K-562 cells incubated with the indicated concentrations of IFN-γ for 24 h.
(F) Cell surface MHC-I in K-562 Cas9 cells expressing control sgRNA or two MTF2-specific sgRNAs incubated ± IFN-γ 10 ng/mL for 24 h.
See also Figure S3.