Extended Data Fig. 1: A C-terminal region in Unc93b1 regulates TLR7 responses.
(a) Unc93b1PKP-expressing macrophages show enhanced TLR7 signaling. Immunoblot of P-p38, P-JNK, P-ERK, and IκBα of RAW macrophages stimulated with R848 (50 ng/ml) for indicated times. Representative of two independent experiments. (b) Unc93b1-Flag expression levels, as measured by Flag immunoblot, of Unc93b1-deficient RAW macrophages retrovirally transduced to express the indicated Unc93b1 alleles. These same cell lines are used for experiments shown in Fig. 1a. All data are representative of three independent experiments, unless otherwise noted.